Composite

Part:BBa_K316027:Design

Designed by: IC 2010 Team   Group: iGEM10_Imperial_College_London   (2010-10-26)

B.subtilis transformation vector with LacI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2922
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1141


Design Notes

Parts were assembled using standard biobrick methods and PCR. Pfu DNA polymerase was used to avoid any errors in replication. Please see our wiki [http://2010.igem.org/Team:Imperial_College_London/Strategy] for details of assembly.


This part is designed for integration into B. subtilis genome at amyE locus, it contains Spectinomycin resistance selection marker and constitutively expresses LacI. PmeI can be used to ligate a gene of interest using blunt ended methods.

Source

Existing biobricks, BBa_K143001, BBa_K316002, BBa_K143053, BBa_K143065, BBa_K143053, BBa_K143062, BBa_K316014 BBa_K143002


References